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5-49.8 °C) and MT (2.7-10.1 N); precise values depended on the species, the individual and the stimulus characteristics. Remote controlled NT threshold testing appears to be a viable refinement for pain research.Metabolic profiling based on 1H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail Helix aspersa. Twenty metabolites were unambiguously identified by 1H, 1D TOCSY, 2D J-resolved, 2D COSY, and 2D HSQC NMR spectra