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Outcomes Respondents had a moderate level of attitude toward PLHIV About 2.00per cent associated with the respondents had a good mindset. Marital status (β=-11.79, p = 0.048) ended up being associated with mindset. Among women, putting on gloves had been connected with mindset (β=5.96, p = 0.041). Conclusions HIV attitude wasn't satisfactory among the HCWs in Isfahan. Therefore, the necessary measures and training are needed to improve the attitudes of wellness personnel and lower