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Primary hCEC cultured on all providers formed a monolayer of tightly loaded cells with a higher mobile viability rate (96% ± 4%). hCEC on HALC and LK20 showed unremarkable phrase of zonula occludens-1 (ZO-1) and Na+/K+-adenosine triphosphatase (ATPase), while Na+/K+-ATPase phrase of cells seeded on dDM was primarily cytoplasmic. All hCEC-carrier constructs had been examined by simulating DMEK surgery in vitro using a person donor cornea without DM mounted on an artificial anterior chamber (AC) and
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